PPARα Agonist WY-14643 Relieves Neuropathic Pain through SIRT1-Mediated Deacetylation of NF-κB

Figure 4

WY-14643 mediated the SIRT1/NF-κB pathway by activating PPARα. (a) The expression of SIRT1 in different groups was detected by qRT-PCR and western blot. WY-14643 administration increased SIRT1 expression. (b) The expression of acetylated NF-κB p65 in different groups was detected by western blot. WY-14643 administration decreased acetylated NF-κB p65 expression, but SIRT1 inhibition increased acetylated NF-κB p65 expression. The relative protein expression was normalized according to the gray value of the band, which was analyzed by ImageJ.

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