Loss of Hepatocyte-Specific PPARγ Expression Ameliorates Early Events of Steatohepatitis in Mice Fed the Methionine and Choline-Deficient Diet

Figure 4

Pparg^ΔHep reduced MCD-induced fibrogenesis. Plasma (a) ALT and (b) AST levels. Hepatic expression of (c) tumor necrosis factor alpha (Tnfa), (d) F4/80, (e) transforming growth factor beta 1 (Tgfb1), (f) alpha smooth muscle actin (aSma), (g) collagen 1a1 (Col1a1), (h), metalloproteinase 13 (Mmp13), and (i) TIMP metallopeptidase inhibitor 1 (Timp1). (j) Quantification of picrosirius red area represented as percentage of red-stained area. (k) 20x representative images of hematoxylin and eosin-stained liver sections. (l) 10x representative images of picrosirius red/fast green-stained liver sections. Values are represented as the . Hepatic gene expression is represented as an absolute mRNA copy number normalized by a normalization factor (NF). Letters represent significant differences between MSD and MCD within groups. Asterisks indicate significant differences between groups within the same diet. ,^A; ,^B; ,^C. Control mice (C); Pparg^ΔHep mice (KO); Pparg^ΔHep mice with hepatocyte Cd36 overexpression (KO+Cd36). MSD diet: open columns, open symbols; MCD diet: close columns, close symbols. mice/group. (k); 100 μM (l).

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