PPARγ Plays an Important Role in Acute Hepatic Ischemia-Reperfusion Injury via AMPK/mTOR Pathway

Figure 4

The influence of PPARγ in IR may be linked with the AMPK/mTOR/autophagy signaling pathway. Notes: (a) the levels of serum ALT and AST for IR after 12 h (, # means for si-PPARγ versus IR, + means for si-PPARγ+Rosi versus si-PPARγ); (b) the levels of serum TNF-α and IL-1β for IR after 12 h (, # means for si-PPARγ versus IR, + means for si-PPARγ+Rosi versus si-PPARγ); (c) protein expression of TNF-α, IL-1β, Bax, and cleaved caspase9 for IR after 12 h (, # means for si-PPARγ versus IR, + means for si-PPARγ+Rosi versus si-PPARγ); (d) TUNEL staining (×200) showed apoptotic cells in mice liver for IR after 12 h; (e) protein expression of AMPK, p-AMPK, mTOR, p-mTOR, LC3, and Beclin1 for IR after 12 h (, # means for IR versus control, + means for IR ± si-PPARγ±Rosi versus IR); (f) immunohistochemistry staining (×200) showing the expression of p-AMPK, p-mTOR, and LC3 for IR after 12 h (, # means for IR versus control, + means for IR ± si-PPARγ±Rosi versus IR).

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