miR-22-3p/PGC1β Suppresses Breast Cancer Cell Tumorigenesis via PPARγ

Figure 4

PGC1β is a direct target of miR-22-3p. (a, c) Putative complementary sites within miR-22-3p and PGC1β predicted by bioinformatics analysis (TargetScan). (b, d) Dual-luciferase reporter assays demonstrated that PGC1β is a direct target of miR-22-3p. (e) PGC1β mRNA level was determined by RT-PCR in MDA-MB-231 and MCF-7 cells with different treatment. (f–h) Representative western blots and quantification of PGC1β and PPARγ in MDA-MB-231 and MCF-7 cells with different treatment. β-Actin was used as an internal control. ; ; .

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