Oleoylethanolamide Alleviates Hepatic Ischemia-Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress-Associated Apoptosis

Figure 5

OEA inhibits ER stress-associated apoptosis to protect liver I/R injury. The mice were treated with TM by intraperitoneal (1 mg/kg) for 24 h, and OEA treatment was conducted for 1 h before the beginning of ischemia. The liver tissue and serum were harvested at 6 h after reperfusion. TM treatment of HepG2 cells (1 μg/ml) was conducted for 6 h, and OEA treatment (20 μM) was conducted for 1 h before OGD/R. The proteins were harvested 6 h after reoxygenation. (a and b) H&E staining and the percentage of necrotic area of the liver section. (c and d) Serum ALT/AST levels. (e and f) Western blotting analysis of PPARα, Grp78, CHOP, Caspase12, and cleaved-Caspase3 expression after OEA or vehicle treatment. (g and h) TM administration for 24 h before ischemia and harvest tissue protein at 6 h after reperfusion. Western blotting analysis of PPARα, Grp78, CHOP, Caspase12, and cleaved-Caspase3 expression alternation. and .

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