Buspirone Induces Weight Loss and Normalization of Blood Pressure via the Stimulation of PPARδ Dependent Energy Producing Pathway in Spontaneously Hypertensive Rats

Figure 4

Estimation of fat cell number in abdominal fat tissues from SHRs treated with buspirone 1, 5, or 10 mg/kg/day (0.01, 0.05, or 0.1 mg/mL/kg/day). Abdominal fat tissue slides of SHRs were fixed by formaldehyde and stained with hematoxylin and eosin dyes (a). Magnification is 100 folds. The number of fat cells per unit square was the largest in 4w, and the number was the least in 8w. The decreased fat cell number was increased by buspirone treatments (b). The results are expressed as mean ± standard error of the mean (). Values were statistically analyzed using the unpaired t-test to compare two groups, and to compare means of three or more groups was used one-way ANOVA test and followed by Bonferroni’s multiple comparisons test. All experiments were repeated over three times. **, ***, ****.

(a)

(b)