Research Article
Appraisal of the Possible Role of PPARγ Upregulation by CLA of Probiotic Pediococcus pentosaceus GS4 in Colon Cancer Mitigation
Figure 9
Effect of biohydrogenation-derived CLA on mitochondrial membrane potential (Ψm): the hHK-II protein was retrieved from protein data bank and molecular docking was performed using AutoDock followed by LigPlot analysis. BE was used in order to rank structures in each cluster. (a) PyMOL view of molecular interaction between c9t11-CLA and hHK-II. (b) LigPlot analysis of c9t11-CLA with the amino acid residues of hHK-II. (c) The HCT-116 cells were pre-treated with BADGE followed by 100 μg/mL CLA/CLAGS4 treatment for 24 hours subsequently Rh 123 staining. The fluorescence intensity was measured using fluorescence spectrophotometry and the data were expressed as % of control. Statistical analysis of data (means ± SE) was determined by one way-ANOVA with post hoc Newman–Keuls analysis and depicted as *versus control; #versus BADGE control.
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