Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain

Figure 3

Production of Leu-encephalin (L-EK) by hBMSCs, pBABE-hBMSCs, and hPPE-hBMSCs was measured for 6 days after gene transfection in vitro (a) and in vivo (b). pBABE, a retroviral vector; hPPE, human proenkephalin; pBABE-hBMSCs, the pBABE-hBMSCs group; hPPE-hBMSCs, the hPPE-hBMSCs group; BCP, bone cancer pain; hPPE, human proenkephalin. In (a), from day 1 to day 6, L-EK levels in hPPE gene engineered hBMSCs group were increasing and significantly higher compared with those in the other two groups. The transfection of hPPE accelerated L-EK secretion from hBMSCs. versus hBMSCs group, versus pBABE-hBMSCs group. In (b), Leu-encephalin (L-EK) expression was significantly upregulated in human bone marrow stem cells (hBMSCs) transfected with hPPE group and the concentration was higher compared with that in pBABE-hBMSCs () and control () groups. The pBABE-hBMSCs group showed a higher concentration than control group (). versus control group; versus control group; versus pBABE-hBMSCs group. .

(a)

(b)