Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain

Figure 4

The development of Walker 256 cell-bearing tibias on the ipsilateral side in rats was shown radiologically (a–d) and by immunohistochemistry (e–h). (a) The normal structure of the rat tibia. (b) The evident periosteal reaction was detected 7 d after cancer cell inoculation. (c) Some loss of medullary bone and the erosion of cortical bone were observed 14 d after injection. (d) Significant cortical bone defects were observed 21 d after cancer cell inoculation. Accordingly, hematoxylin and eosin (HE) staining of the tumor area in the rat ipsilateral tibia on different days after operation is shown (e–h). Scale: 50 mm, .

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