Systematic Administration of B Vitamins Alleviates Diabetic Pain and Inhibits Associated Expression of P2X3 and TRPV1 in Dorsal Root Ganglion Neurons and Proinflammatory Cytokines in Spinal Cord in Rats

Figure 2

Mechanical allodynia and thermal hyperalgesia after intraperitoneal injection of streptozotocin (STZ) and effects of VBC treatment on the allodynia and hyperalgesia. Development of STZ-induced mechanical hypersensitivity (allodynia) manifested as significant decrease of the mechanical withdrawal thresholds (a) and thermal hyperalgesia manifested as significant shortened latency of thermal withdrawal (b) and long-lasting inhibitory effects of i.p. VBC on the allodynia and thermal hyperalgesia. Immediate inhibitory effects of VBC on STZ-induced mechanical allodynia (c) and thermal hyperalgesia (d). Numbers of animals corresponding to testing day in each group are indicated in the parentheses. Two-way ANOVA, versus control. Student’s t-test, ; vs. the testing point on day 35 after STZ (a, b) and immediately before the first dose of VBC treatment at point zero (c, d).

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(b)

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