miR-223 Inhibits the Polarization and Recruitment of Macrophages via NLRP3/IL-1β Pathway to Meliorate Neuropathic Pain

Figure 4

The effect of miR-223 inhibition on macrophage subsets in injured peripheral nerves. (a) Chemotaxis experiment of RAW264.7 and glial cells derived from mice injected with Lenti-vector or Lenti-miR-223. Cell count staining showed that LPS-treated microglial cells had a significant recruitment effect on RAW264.7 cells, while overexpression of miR-223 significantly reduced the recruitment effect. (b) Cells were isolated from peripheral nerves, and then, macrophages were selected based on the expression of CD45 and CD11b. Among CD45+ and CD11b + macrophages, they were analyzed for the expression of CD86 and CD206. (c) Compared with the sham group, the total number of macrophages in each group was significantly increased, while the overexpression of miR-223 significantly inhibited the recruitment of macrophages. n = 6. , a significant statistical difference compared with the sham group. ns, no significant statistical difference compared with the CCI group. , a significant statistical difference compared with the CCI + Lenti-vector group. (d) The number of CD86 positive cells in each group. CD86 positive cells in the CCI group increased significantly. miR-223 overexpression significantly reduced the number of CD86 positive cells. n = 6. , a significant statistical difference compared with the sham group. ns, no significant statistical difference compared with the CCI group. , a significant statistical difference compared with the CCI + Lenti-vector group. (e) The number of CD206 positive cells in each group; compared to the CCI group, overexpression of miR-223 significantly increased the number of CD206+ positive macrophages cells. n = 6. , a significant statistical difference compared with the sham group; ns, no significant statistical difference compare with the CCI group. , a significant statistical difference compared with the CCI + Lenti-vector group.

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