Abdominal Massage Improves the Symptoms of Irritable Bowel Syndrome by Regulating Mast Cells via the Trypase-PAR2-PKCε Pathway in Rats

Figure 4

Abdominal massage attenuated IBS-induced activation of the trypase-PAR2-PKCε pathway. (a) Representative images of trypase IHC staining (left panel) and trypase AOD value analyzed with ImageJ software (right panel). (b) Detection of PAR2 and PKC protein levels in small intestinal mucosa tissues from the normal, IBS, abdominal massage, and ketotifen treatment groups by western blot (left panel: representative images of PAR2 and PKC western blot; middle panel, a summary of relative PKC levels in four groups; right panel, a summary of relative protein levels of PAR2 in four groups. Data were normalized to GAPDH). (c) Detection of PLC and PIP2 protein levels in small intestinal mucosa tissues from the normal, IBS, abdominal massage, and ketotifen treatment groups by western blot (right panel: representative blots of PLC and PIP2; middle panel, a summary of relative PLC levels in four groups; right panel, a summary of relative protein levels of PIP2 in four groups. Data were normalized to GAPDH). , , .

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