Research Article

Histochemical Comparison of the Hypopharyngeal Gland in Apis cerana Fabricius, 1793 Workers and Apis mellifera Linnaeus, 1758 Workers

Figure 3

Light microscope micrographs of hypopharyngeal gland acini stained with PAS. Abbreveations: bm: basement membrane; ex: extracellular space; n: nucleus; sc: secretory unit; sd: secretory duct; vs: vesicle.
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(a) High-resolution micrograph of the A. cerana nurse bee hypopharyngeal gland stained pink-purple surrounding the secretory vesicles (1000x)
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(b) A section of the hypopharyngeal gland of an A. mellifera worker nurse bee showing the cytoplasm of the secretory cell containing a variable number of secretory vesicles which is stained red-pink with PAS. The oval nucleus stains pale greenish with light green (1000x)
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(c) A medial section of the completely developed acinar gland of A. cerana nurse bee showing a cluster of several secretory units. The secretory cell cytoplasm is stained pink with PAS and the cell has a large nucleus. (100x)
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(d) The secretory units of the hypopharyngeal gland of A. mellifera forager showing the smaller size of secretory vesicles containing less carbohydrate. These are characterized by staining slightly red-pink with PAS and showing the wide extracellular space between adjacent acinar cells which is seen by white color separating it from the other secretory cells (100x)
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(e) With a higher magnification of A. mellifera guard, the secretory cell contains various sizes of secretory vesicles which are less stained to PAS than that of nurse bee (200x)
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(f) A medial section of the hypopharyngeal gland of an A. cerana guard showing the shrinkage and damaged plasma membrane (400x)