Sister Chromatid Exchange and Genomic Instability in Soft Tissue Sarcomas: Potential Implications for Response to DNA-Damaging Treatments

Figure 1

Endogenous sister chromatid exchanges and γH2AX foci in sarcoma cells. (a) Harlequin-stained metaphase chromosomes from a Shef-DDLPS 02 (dedifferentiated liposarcoma) cell showing a hyperdiploid karyotype with over 120 chromosomes. (b) Higher magnification of a section of the same metaphase chromosome spread showing nine sister chromatid exchanges (red arrows). (c) Interphase nuclei of hTERT-RPE1 (human retinal epithelium) cells showing <10 endogenous γH2AX foci each (red dots). (d) Shef-DDLPS 02 cell nucleus showing >10 γH2AX foci (red dots). Metaphase chromosomes were stained with Hoechst 33258 dye followed by exposure to UV light. Interphase nuclei were stained using Cy3-conjugated rabbit anti-γH2AX antibody and counterstained blue with DAPI.

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