Nanoscale Features of Gambogic Acid Induced ROS-Dependent Apoptosis in Esophageal Cancer Cells Imaged by Atomic Force Microscopy

Figure 4

Effects of gambogic acid on apoptosis of EC9706 cells after 24 h treatment. Intracellular ROS level of (a) control, (b) 0.4 μM gambogic acid-treated, (c) 1.2 μM gambogic acid-treated, (d) 2 μM gambogic acid-treated, (e) 5 mM NAC+2 μM gambogic acid-treated, and (f) 5 mM NAC-treated EC9706 cells. EC9706 cells in (e, f) were pretreated with 5 mM NAC for 1 h and then treated with or without gambogic acid for 24 h to determine the effects of ROS scavenger-NAC on gambogic acid induced apoptosis in EC9706 cells.

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