Nanoscale Features of Gambogic Acid Induced ROS-Dependent Apoptosis in Esophageal Cancer Cells Imaged by Atomic Force Microscopy

Figure 7

Effects of gambogic acid on cell morphology of EC9706 cells after 24 h treatment. AFM morphology imaging of (a) control, (b) 0.4 μM gambogic acid-treated, (c) 1.2 μM gambogic acid-treated, (d) 2 μM gambogic acid-treated, (e) 5 mM NAC+2 μM gambogic acid-treated, and (f) 5 mM NAC-treated EC9706 cells. (A1-F1) Topography images and (A2-F2) their corresponding deflection error images. (A3-F3) Enlarged topography images as indicated by the blue frames in (A1-F1) and (A4-F4) their corresponding deflection error images. Scale bar: 20 μm. EC9706 cells in (e, f) were pretreated with 5 mM NAC for 1 h and then treated with or without gambogic acid for 24 h to determine the effects of ROS scavenger-NAC on gambogic acid induced morphological damage in EC9706 cells by AFM imaging in air with contact mode.