Helium Ion Microscopy and Sectioning of Spider Silk

Figure 1

HIM images of a MAS fiber show the effect of increasing milling overtime in the marked area with an ion dose of 0.5nC/μm² between each successive image. It can be seen that the outer layers are etched away by the sputtering of He⁺ ions, and the inner structure of fibrils becomes visible. The scale bar is 1 μm.

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