Morphological Characteristics of Young and Old Murine Hematopoietic Stem Cell Niches, as Modeled In Vitro

Figure 2

Preparation of an artificial hematopoietic stem cell (HSC) niche. First, the bone crush method is used to isolate BM cells from young and old mice. The cells are then separately cultivated in a commercial MyeloCult medium for 1-3 weeks and regularly checked with light microscopy and SEM.