Characterization of Nora Virus Structural Proteins via Western Blot Analysis

Figure 2

SDS-PAGE analysis of recombinant Nora virus proteins (rNVPs). Recombinant Nora virus proteins were produced by overexpression in E. coli cells as described in Section 2. The resultant proteins were purified using Ni⁺² charged IMAC columns either as individual proteins (lanes labeled VP3, VP4a, VP4b, VP4c, and ORF1) or as a mixture of the 5 proteins (lanes labeled rVirus). The proteins were subsequently separated on 10% SDS-PAGE gels followed by Coomassie blue staining. The results showed the relative purity of the preparations and demonstrated that the proteins readily copurified when mixed together.