Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi

Figure 3

R. equi infection of BMDM leads to autophagy induction. (a) Western blotting analysis showing the expression of LC3-II levels in the whole cell protein lysates of BMDM infected with R. equi (moi3). LC3-II expression was observed at 24 h and 48 h. β-Actin immunoblot represents equal protein loading. Densitometry analysis was used to quantify the density of LC3-II bands. The ratio of LC3-II bands to that of β-actin was determined and the data are expressed as percent of control. Data represent mean ± SEM, compared with untreated group. (b) Following infection of BMDM with R. equi (moi3), cells were stained with MDC and the fluorescence was measured by a plate reader. A marked increase in MDC staining was evidenced in BMDM at 24 h and 48 h of R. equi infection. The results are expressed as mean ± SEM of 6 independent measurements. ; .

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