The Optimization of Medium Conditions and Auxins in the Induction of Adventitious Roots of Pokeweed (Phytolacca americana L.) and Their Phytochemical Constituents

Table 1

Pokeweed adventitious root induction percentages and number of adventitious and lateral roots influenced by medium state and supplemented PGRs.

Treatment	Adventitious root induction percentage (%)		Number of adventitious roots (roots per explant)		Number of lateral roots (roots per explant)
	Semisolid	Liquid	Semisolid	Liquid	Semisolid	Liquid
Control	0.00 ± 0.00^f	0.00 ± 0.00^f	0.00 ± 0.00^e	0.00 ± 0.00^e	0.00 ± 0.00^d	0.00 ± 0.00^d
NAA (mg/L)
0.5	75.00 ± 17.08^b–d	83.33 ± 10.54^a–c	3.00 ± 0.36^b–d	6.00 ± 0.89^a	12.67 ± 1.74^a	2.33 ± 0.71^c
1	91.67 ± 8.33^ab	100.00 ± 0.00^a	4.33 ± 0.80^b	5.83 ± 0.65^a	6.50 ± 0.99^b	1.50 ± 0.55^cd
2	83.33 ± 10.54^a–c	50.00 ± 0.00^e	2.83 ± 0.48^cd	6.17 ± 0.70^a	7.33 ± 0.80^b	0.00 ± 0.00^d
4	91.67 ± 8.33^ab	0.00 ± 0.00^f	2.83 ± 0.65^cd	0.00 ± 0.00^e	8.16 ± 1.01^b	0.00 ± 0.00^d
IBA (mg/L)
0.5	66.67 ± 10.54^c–e	0.00 ± 0.00^f	2.67 ± 0.56^d	0.00 ± 0.00^e	1.67 ± 0.49^cd	0.00 ± 0.00^d
1	66.67 ± 10.54^c–e	0.00 ± 0.00^f	4.17 ± 0.60^b	0.00 ± 0.00^e	2.17 ± 0.60^c	0.00 ± 0.00^d
2	58.33 ± 8.33^de	0.00 ± 0.00^f	2.67 ± 0.71^d	0.00 ± 0.00^e	1.33 ± 0.21^cd	0.00 ± 0.00^d
4	58.33 ± 15.37^de	0.00 ± 0.00^f	2.83 ± 0.60^cd	0.00 ± 0.00^e	1.83 ± 0.15^c	0.00 ± 0.00^d

Data are expressed as mean ± SE. Significant differences between means were determined by two-way ANOVA and the least significant difference test (). Different letters (a, b, c, …) represent significant differences in both semisolid and liquid columns ().