Method to Determine the Optimal Aptamer-to-Bead Ratio by Using Flow Cytometry

<div>Flow cytometry analysis of aptamer-bead complexes. Results of the analysis of 1 × 10<sup>4</sup> beads after interaction with the aptamer or fluorescent aptamer. (a) Scatter plot (A) and histogram (B) of the aptamer-bead complex without fluorescent labeling. FSC: forward scatter, SSC: side scatter, <i>K</i>: × 10<sup>3</sup>. (b) Comparative histogram of beads that had interacted with the aptamer or fluorescent aptamer. FAM: 6-carboxyfluorescein.</div>

Scientifica

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Figure 1

Figure 1: Method to Determine the Optimal Aptamer-to-Bead Ratio by Using Flow Cytometry 