Method to Determine the Optimal Aptamer-to-Bead Ratio by Using Flow Cytometry

Figure 3

Confirmation of aptamer-to-bead ratio by fluorescence. (a) Fluorescence microscopy analysis of beads coupled with aptamer_FAM at ratios gradually increasing from 0 to 256 × 10³ (scale bar = 20 μm). Con: light microscopy, FAM: fluorescence microscopy (green). (b) Bar graphs representing the fluorescence in (a). Photomicrographs were taken at 200× magnification. “0” indicates control without aptamer. All experiments were repeated three times. Error bars represent standard deviations of replicates (n = 3). Statistically significant difference in fluorescence intensity compared to control ( value <0.001). (c) Bar graph representing the rate of fluorescence increase obtained by dividing the average of the indicated ratio by the previous average fluorescence. The statistically significant difference in fluorescence intensity compared to ratio 32 × 10³. ,,. Without a star, no statistical significance.

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