Research Article
Method to Determine the Optimal Aptamer-to-Bead Ratio by Using Flow Cytometry
Figure 4
Determination of the most efficient aptamer-to-bead ratio by varying the free aptamer concentration. (a) Analysis of the amount of free aptamer by fluorescence spectrometry. “0” indicates control without aptamer. Error bars represent standard deviations of replicates (n = 3). Statistically significant difference in fluorescence intensity compared to control ( value <0.001). (b) Bar graph representing the rate of fluorescence increase measured under the same conditions as in Figure 2(c). The statistically significant difference in fluorescence intensity compared to the ratio 1 × 103. ,. Without a star, no statistical significance.
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