Method to Determine the Optimal Aptamer-to-Bead Ratio by Using Flow Cytometry

Figure 5

Verification of aptamer Co²⁺ binding capacity at each aptamer-to-bead ratio by ICP/OES analysis. The concentration of Co²⁺ ions was measured by ICP/OES after exposure to the indicated aptamer-bead complex. All experiments were repeated three times. The removal rate is expressed as a percentage (passing through/initial ion concentration). Error bars represent standard deviations of replicates (n = 3). Statistically significant difference in removal rate compared to control ( value <0.001).