Research Article
Development and Validation of Gene-Based SSR Markers in the Genus Mesembryanthemum
Table 1
Expressed sequence tag-simple sequence repeats (EST-SSRs) frequencies by repeat motif in Mesembryanthemum.
| | SSR motif | 5 | 6 | 7 | 8 | 9 | 10 | 11–20 | 21–30 | 31–40 | 41–50 | >50 | Total |
| | A/T | 0 | 0 | 0 | 0 | 0 | 899 | 1567 | 257 | 42 | 20 | 28 | 2813 | | C/G | 0 | 0 | 0 | 0 | 0 | 112 | 202 | 11 | 4 | 0 | 1 | 330 | | AC/GT | 0 | 107 | 9 | 6 | 1 | 3 | 4 | 0 | 0 | 0 | 0 | 130 | | AG/CT | 0 | 971 | 286 | 191 | 214 | 154 | 386 | 52 | 37 | 14 | 14 | 2319 | | AT/AT | 0 | 134 | 51 | 5 | 3 | 2 | 30 | 7 | 1 | 0 | 0 | 233 | | CG/CG | 0 | 2 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 6 | | AAC/GTT | 64 | 22 | 10 | 9 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 116 | | AAG/CTT | 119 | 58 | 45 | 16 | 6 | 7 | 10 | 0 | 0 | 0 | 0 | 261 | | AAT/ATT | 14 | 130 | 13 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 160 | | ACC/GGT | 71 | 102 | 9 | 29 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 219 | | ACG/CGT | 11 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 18 | | ACT/AGT | 13 | 3 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 19 | | AGC/CTG | 31 | 16 | 10 | 25 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 85 | | AGG/CCT | 24 | 9 | 8 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 42 | | ATC/ATG | 116 | 39 | 25 | 5 | 17 | 11 | 9 | 0 | 0 | 0 | 0 | 222 | | CCG/CGG | 31 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 35 | | AAAC/GTTT | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AAAG/CTTT | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | | AAAT/ATTT | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | | AAGG/CCTT | 4 | 5 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 18 | | AATC/ATTG | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | AATT/AATT | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | ACAT/ATGT | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | | ACTC/AGTG | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | | AGAT/ATCT | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AGCC/CTGG | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AGGG/CCCT | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | ATGC/ATGC | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AAAAG/CTTTT | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | | AAATC/ATTTG | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AAGAG/CTCTT | 0 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | | AATCG/ATTCG | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | ACAGC/CTGTG | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | ACCTC/AGGTG | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | ACTCT/AGAGT | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AGAGG/CCTCT | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | AGGGG/CCCCT | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | | ATATC/ATATG | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | ATCCG/ATCGG | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | AAAATC/ATTTTG | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AAAAAG/CTTTTT | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AAAGAC/CTTTGT | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AAAGAG/CTCTTT | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | AAATGC/ATTTGC | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AAATTG/AATTTC | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | | AACACC/GGTGTT | 6 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | | AACAGC/CTGTTG | 22 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 25 | | AACATC/ATGTTG | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AACCAC/GGTTGT | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AACCAT/ATGGTT | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | | AACTAC/AGTTGT | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | AACTGC/AGTTGC | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | AAGAGG/CCTCTT | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | | AAGATG/ATCTTC | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | | AAGGAC/CCTTGT | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | AATCCC/ATTGGG | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AATTAC/AATTGT | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | ACACAT/ATGTGT | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | ACAGGG/CCCTGT | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | ACCCTG/AGGGTC | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AGAGAT/ATCTCT | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AGAGGG/CCCTCT | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | | AGCAGG/CCTGCT | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | | AGGATG/ATCCTC | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | | AGGGAT/ATCCCT | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
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