Development and Validation of Gene-Based SSR Markers in the Genus Mesembryanthemum

Table 6

Number of alleles, size range of amplified fragments, and polymorphism statistics calculated with iMEC for 24 Mesembryanthemum genotypes using 21 expressed sequence tag-simple sequence repeat (EST-SSR) loci.

Locus	Number of alleles	Size range (bp)	Polymorphic information content (PIC)	Heterozygosity index (H)	Average heterozygosity (av. H)	Marker index (MI)	Discriminating power (D)
PMA5	2	850–1100	0.47	0.38	0.38	0.38	0.94
PMA11	5	167–2000	0.43	0.47	0.47	0.47	0.62
PMA20	1	248	0.47	0.38	0.38	0.38	0.95
PMA21	4	400–950	0.51	0.36	0.36	0.36	0.34
PMA23	1	300	0.53	0.16	0.16	0.16	0.16
PMA29	3	250–700	0.47	0.61	0.61	0.61	0.52
PMA30	1	200	0.54	0.08	0.08	0.08	1
PMA32	5	250–750	0.42	0.5	0.5	0.5	0.8
PMA38	1	244	0.53	0.15	0.15	0.15	1
PMA40	2	230–305	0.41	0.5	0.5	0.5	0.73
PMA44	1	297	0.54	0	0	0	0
PMA48	5	350–1000	0.43	0.47	0.47	0.47	0.86
PMA57	4	350–1000	0.48	0.54	0.54	0.54	0.54
PMA62	1	500	0.46	0.47	0.47	0.47	0.49
PMA63	2	120–800	0.46	0.66	0.66	0.66	0.63
PMA64	5	212–900	0.41	0.5	0.5	0.5	0.74
PMA67	4	233–650	0.42	0.48	0.48	0.48	0.84
PMA76	3	150–400	0.43	0.47	0.47	0.47	0.86
PMA77	4	350–750	0.45	0.41	0.41	0.41	0.92
PMA79	6	250–900	0.42	0.5	0.5	0.5	0.78
PMA80	3	600–800	0.44	0.51	0.51	0.51	0.38
PMA83	2	500–600	0.43	0.48	0.48	0.48	0.64