Estimating the Genetic Diversity of Oranges Citrus spp. in South Sulawesi, Indonesia, Using RAPD Markers

Table 3

Details of RAPD primers and obtained polymorphisms.

Primer	Primer sequences (5′-3′)	Ta (°C)	Fragment size (bp)	Number of band	Number of fragment polymorphic	% fragment polymorphic	PIC
OPE-04	GTG ACA TGC C	30.4	400–1300 bp	5	5	100	0.378
OPH-04	GGA AGT CGC C	40.3	250–1600 bp	6	6	100	0.388
OPH-15	AAT GGC GCA G	35.4	200–1200 bp	12	12	100	0.196
OPN-14	TCG TGC GGG T	43.8	200–1800 bp	7	7	100	0.258
OPN-16	AAG CGA CCT G	34.5	250–1100 bp	9	9	100	0.291
OPR-08	CCA TTC CCC A	33.8	300–2000 bp	8	8	100	0.192
OPR-20	TCG GCA CGC A	45.1	250–1200 bp	10	10	100	0.178
OPW-06	AGG CCC GAT G	37.6	250–1400 bp	9	9	100	0.274
OPW-09	GTG ACC GAG T	37.6	200–700 bp	7	7	100	0.220
OPX-07	GAG CGA GGC T	41.2	200–1600 bp	8	8	100	0.288
OPX-11	GGA GCC TCA G	36.0	350–1000 bp	8	8	100	0.143
OPX-17	GAC ACG GAC C	36.2	350–1000 bp	4	4	100	0.350
UBC-18	GGG CCG TTT A	32.3	350–1000 bp	5	5	100	0.198
UBC-51	CTA CCC GTG C	41.3	200–1500 bp	11	11	100	0.188
Total				109	109		3.542
Mean						100	0.253