rmvPFBAM: Removing Primers from BAM Files Based on Amplicon-Based Next-Generation Sequencing and Cloud Computing When Analyzing Personal Genome Data

Figure 1

Scheme of the target sequencing by multiplex PCR. The blue region is the target region that we want to get the sequence of that and millions of reads are produced to cover it. For each read, the red sequence is the forward primer and the green sequence is the reverse primer. For each pair of read, read 1 contains forward primer (red) and read 2 contains reverse primer (green).