LncRNA NKILA Promotes Cardiomyocytes Apoptosis by Targeting miR22-3p-TXNIP Signal Axis to Inhibit Proliferation, Migration, and Invasion of Cardiomyocytes under High Glucose-Induced Condition

Figure 2

Knocking down LncRNA NKILA reversed the apoptosis of AC16 cells induced by high glucose and promoted its proliferation. (a) AC16 cells viability detection by CCK8 assay of knocking down LncRNA NKILA under high glucose-induced condition and indicated plasmids were transfected in AC16 cells; (b, c) AC16 cells colony forming ability and proliferation ability detection by colony forming assay and EDU assay of knocking down LncRNA NKILA under high glucose-induced condition and indicated plasmids were transfected in AC16 cells; (d) AC16 cells apoptosis detection by Annexin V-FITC assay of knocking down LncRNA NKILA under high glucose-induced condition and indicated plasmids were transfected in AC16 cells; (e) cell apoptosis protein makers expression level detection by western blot assay after transfecting indicated plasmids in AC16 cells and indicated antibodies were added during western blot assay. Data are representative of three independent experiments and were analyzed by the unpaired t-test. Error bars denote SD.; ; .

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