LncRNA NKILA Promotes Cardiomyocytes Apoptosis by Targeting miR22-3p-TXNIP Signal Axis to Inhibit Proliferation, Migration, and Invasion of Cardiomyocytes under High Glucose-Induced Condition

Figure 4

LncRNA NKILA in regulating AC16 cells proliferation, migration, and invasion. (a) Under high glucose-induced condition, the expression level of LncRNA NKILA mRNA in AC16 cells. Nucleus and cytoplasm was detected by RT-PCR after nuclear and cytoplasmic separation assay; (b) aligned region between sequence of LncRNA NKILA and miR22-3p and sequence of LncRNA NKILA mutation (NKILA Mut); (c) luciferase activity assay to analyze the interaction between LncRNA NKILA (wt: wild type or Mut: mutation) and miR22-3p under high glucose-induced condition; (d) the expression level of miR22-3p mRNA detected by RT-PCR after inducing on 30 mM glucose at the indicated time in AC16 cells; (e) the expression level of miR22-3p mRNA detected by RT-PCR after inducing with various concentrations of glucose (0, 5, 10, 20, and 30 mM) for 48 h in AC16 cells; (f) the expression level of miR22-3p mRNA detected by RT-PCR after transfecting indicated plasmid in AC16 cells under high glucose-induced condition. Data are representative of three independent experiments and were analyzed by the unpaired t-test. Error bars denote SD.; ; .

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