LncRNA NKILA Promotes Cardiomyocytes Apoptosis by Targeting miR22-3p-TXNIP Signal Axis to Inhibit Proliferation, Migration, and Invasion of Cardiomyocytes under High Glucose-Induced Condition

Figure 5

The influence of miR22-3p on AC16 cells proliferation, migration, and invasion under high glucose-induced condition. (a) Under high glucose-induced condition, the expression level of miR22-3p mRNA in AC16 cells was detected by RT-PCR after indicated plasmids were transfected in AC16 cells; (b) AC16 cell viability detection by CCK8 assay under high glucose-induced condition after indicated plasmids were transfected in AC16 cells; (c, d) AC16 cells colony forming ability and proliferation ability detection by colony forming assay and EDU assay of knocking down LncRNA NKILA under high glucose-induced condition, and indicated plasmids were transfected in AC16 cells; (e) AC16 cells apoptosis detection by Annexin V-FITC assay under high glucose-induced condition after indicated plasmids were transfected in AC16 cells; (f) cell apoptosis protein makers expression level detection by western blot assay after transfecting indicated plasmids in AC16 cells and indicated antibodies were added during western blot assay. Data are representative of three independent experiments and were analyzed by the unpaired t-test. Error bars denote SD.; ; .

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