LncRNA NKILA Promotes Cardiomyocytes Apoptosis by Targeting miR22-3p-TXNIP Signal Axis to Inhibit Proliferation, Migration, and Invasion of Cardiomyocytes under High Glucose-Induced Condition

Figure 7

TXNIP is a target of miR-22-3p in AC16 cells. (a) Aligned region between sequence of TXNIP and miR22-3p and sequence of TXNIP mutation(TXNIP Mut); (b) luciferase activity assay to analyze the interaction between TXNIP (wt: wild type or Mut: mutation) and miR22-3p, under high glucose-induced condition; (c) the expression level of TXNIP mRNA detected by RT-PCR after inducing on 30 mM glucose at the indicated time in AC16 cells; (d) the expression level of TXNIP mRNA detected by RT-PCR after inducing with various concentrations of glucose (0, 5, 10, 20, and 30 mM) for 48 h in AC16 cells; (e, f) TXNIP mRNA and protein expression level inhibited by miR22-3p mRNA, mRNA, and protein expression level were detected by RT-PCR and western blot assay after transfecting indicated plasmids in AC16 cells under high glucose-induced condition and indicated antibodies were added during western blot assay. Data are representative of three independent experiments and were analyzed by the unpaired t-test. Error bars denote SD.; ; .

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