LncRNA NKILA Promotes Cardiomyocytes Apoptosis by Targeting miR22-3p-TXNIP Signal Axis to Inhibit Proliferation, Migration, and Invasion of Cardiomyocytes under High Glucose-Induced Condition

Figure 8

LncRNA NKILA performed its function in AC16 cells under high glucose-induced condition by targeting mir-22-3p-TXNIP signal axis. (a) LncRNA NKILA mRNA expression level was detected by RT-PCR after transfecting indicated plasmids in AC16 cells under high glucose (b) and (c) AC16 cell viability and proliferation ability detection by CCK8 assay and EDU assay under high glucose=induced condition after indicated plasmids were transfected in AC16 cells; (d) AC16 cells apoptosis detection by Annexin V-FITC assay under high glucose-induced condition after indicated plasmids were transfected in AC16 cells; (e, f) AC16 cells migration and invasion detection by the wound healing test and transwall chamber assay under high glucose-induced condition after transfecting indicated plasmids in AC16 cells. Data are representative of three independent experiments and were analyzed by the unpaired t-test. Error bars denote SD.; ; .

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