Stroke Status Evoked Adhesion Molecule Genetic Alterations in Astrocytes Isolated from Stroke-Prone Spontaneously Hypertensive Rats and the Apigenin Inhibition of Their Expression

Figure 3

The effects of apigenin and resveratrol on the TNF-$\alpha$-induced expression of ICAM-1, MCP-1, and VCAM-1 in astrocytes isolated from SHRSP/IZM. Time course: the astrocytes isolated from SHRSP/IZM were washed with FBS-free DMEM, before (10 $\mu$M) TNF-$\alpha$ and apigenin or resveratrol were added. The astrocytes were cultured for 1, 2, or 4 hours. The ICAM-1(a), MCP-1 (b), and VCAM-1 (c) mRNA measurements were carried out as described in Figure 1 and in the text. The bars represent the mean ± SD (n = 4). Asterisk (*): $P<.05$ compared with the expression level of TNF-$\alpha$ value at the same time. +Api; apigenin, +Res: resveratrol.

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