Detection and Characterization of Goose Astrovirus Infections in Hatcheries and Commercial Goose Flocks

Figure 2

Phylogenetic analysis of isolates in this study. (a) Phylogenetic analysis of the RdRp genes of astroviruses using MEGA 7.0 software. The tree was constructed based on about 1500 nt (nucleotide) sequences using the neighbor-joining method with 1000 bootstrap replicates and the maximum composite likelihood model. Node labels indicated bootstrap values, and bootstrap values <50% were hidden. The strains isolated in this study are indicated by a black triangle and strain SD01 is indicated by a red triangle. (b) Phylogenetic analysis of goose astrovirus genotype G2. Phylogenetic relationship analysis was based on the amino acid sequences of ORF2. The trees were generated using MEGA 7.0 software and the neighbor-joining method with 1000 bootstrap replicates and the evolutionary distances were computed using the JTT matrix-based method. The strains isolated in this study are indicated by a black triangle and four representative isolates are indicated by a red triangle.

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