Genetic Evolution and Biological Characteristics of Feline Caliciviruses Isolated from Dogs

Figure 1

Isolation and identification of two FCV strains from dogs. (a) RT-PCR with 28F/413R primer sets. M: Marker (Takara DNA Ladder 2000); Line 1–6: PCR products of six FCV-positive samples from dogs of 380 bp in size obtained using feline calicivirus primers. Line 7: positive control. Line 8: negative control. (b) Cytopathic effects (CPEs) of two canine-origin FCV isolates on CRFK and MDCK cells. CRFK and MDCK cells were inoculated with two isolates (canine/GXHC01–21 and canine/GXNN10-21) and negative medium, as shown at 12 hours post-infection. Magnification, 200×. (c) Indirect immunofluorescence analysis on CRFK cells. CRFK cells were inoculated with the two strains of canine viruses at a multiplicity of infection of 0.01 and observed at 12 hours post-infection. The green fluorescence on CRFK cells was revealed by using a cat polyclonal serum against the FCV GX01-13 strain. The CPE were observed using white light and the nuclei strained by DAPI are shown as blue.

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