Genetic Evolution and Biological Characteristics of Feline Caliciviruses Isolated from Dogs

Figure 2

Phylogenetic trees of two canine-origin FCV isolates, 11 previous FCV isolates and referenced strains available from GenBank based on the complete genome (a) and the nucleotides sequences of the ORF2 genes (b). Phylogenetic trees were generated by using the maximum likelihood (ML) method with the Tamura-Nei (a) and Kimura 2-parameter (b) models. A value ≥ 70 for the ML bootstrap (1000 replications) is shown at the main branch of the trees. The two canine-origin isolates obtained in this study are marked with “red triangles”; The FCV strain canine/213/95 isolated from Italy [24] is indicated with a “black diamond”; The commonly used vaccine strains are indicated with “black squares”; Previous published FCV isolates from the respiratory and enteric tracts are marked with “hollow circles and triangles”, respectively.

(a)

(b)