Genetic Evolution and Biological Characteristics of Feline Caliciviruses Isolated from Dogs

Figure 4

Comparative growth properties between the two canine-origin FCV isolates, R-FCV (GX01-13) and E-FCV (GXNN04-20). (a) One-step growth curves of viruses in CRFK and MDCK cells at a multiplicity of infection of 5 (^∗ < 0.05; ^∗∗ < 0.01; ^∗∗∗ < 0.001; ^∗∗∗∗ < 0.0001). (b) Plaque phenotype in monolayer CRFK cells were inoculated with 10-fold serial dilutions of each FCV strain at 37°C for 1 h. Infected cells were incubated for 36 hours post-infection, before staining with 0.8% (w/v) crystal violet solution. (c) The average plaque areas for the four FCV strains were measured and calculated using ImageJ software. Statistical analysis was performed by three-way ANOVA.

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