Transboundary and Emerging Diseases

Research Article

Evaluation of the Diagnostic Sensitivity of the VIASURE Leishmania Real-Time PCR Detection Kit Prototype for the Diagnosis of Cutaneous and Visceral Leishmaniasis

Table 2

Clinical performance based on analysis of DNA samples (n = 49): mean Ct values of the duplicate samples for each qPCR kit.


Sample ID	Culture	VIASURE Ct	SACACE Ct	Clinical form/sample	Species identification	Sample ID	Culture	VIASURE Ct	SACACE Ct	Clinical form/sample	Species identification

1	Neg	36.944	35.852	CL/SB		26	Neg	28.818	28.950	CL/SB
2	Neg	Neg	Neg	CL/SB		27	Neg	Neg	Neg	CL/SB
3	Neg	Neg	Neg	CL/SB		28	Neg	36.274	35.693	CL/SB
4	Neg	Neg	Neg	CL/SB		29	Neg	Neg	Neg	CL/SB
5	Neg	Neg	Neg	CL/SB		30	Pos	16.299	16.664	CL/SB	L. infantum
6	Neg	Neg	Neg	CL/SB		31	Neg	32.437	33.767	CL/SB
7	Pos	21.028	19.867	CL/SB	L. braziliensis	32	Neg	33.370	34.922	CL/SB
8	Neg	31.401	31.326	CL/SB		33	Neg	38.231	38.497	CL/SB
9	Neg	Neg	Neg	CL/SB		34	Pos	33.476	32.940	CL/SB	L. panamensis
10	Neg	32.812	33.210	CL/SB		35	Neg	Neg	37.799	CL/SB
11	Neg	Neg	37.669	CL/SB		36	Neg	Neg	Neg	CL/SB
12	Pos	20.922	23.568	CL/SB	L. infantum	37	Neg	33.867	31.056	CL/SB
13	Pos	18.029	21.003	CL/SB	L. infantum	38	Neg	Neg	Neg	CL/SB
14	Neg	39.649	36.799	CL/SB		39	Neg	Neg	Neg	CL/SB
15	Pos	24.630	25.873	CL/SB	L. infantum	40	Neg	Neg	Neg	CL/SB
16	Pos	28.131	28.439	CL/SB	L. major	41	Pos	26.841	29.345	CL/SB	L. infantum
17	Neg	Neg	Neg	CL/SB		42	Neg	Neg	Neg	CL/SB
18	Pos	27.005	27.643	CL/SB	L. major	43	Neg	Neg	Neg	CL/SB
19	Neg	Neg	36.215	CL/SB		44	Neg	Neg	Neg	CL/SB
20	Pos	31.950	30.948	CL/SB	L. major	45	Neg	26.485	26.871	VL/LB
21	Neg	Neg	37.315	CL/SB		46	Pos	22.668	22.829	VL/BM	L. infantum
22	Neg	Neg	37.171	CL/SB		47	Pos	29.201	27.664	VL/BM	L. infantum
23	Neg	34.787	32.655	CL/SB		48	Pos	32.353	32.874	VL/PB	L. infantum
24	Neg	29.512	28.445	CL/SB		49	Neg	Neg	36.435	VL/BM
25	Neg	25.604	23.391	CL/SB		Total POS	13/49	27/49	33/49

CL: cutaneous leishmaniasis, VL: visceral leishmaniasis, SB: skin biopsy, BM: bone marrow, LB: liver biopsy, PB: peripheral blood; Positive result by the STAT-NAT® Leishmania spp. Kit (Sentinel, Milano, Italy). Characterization was done by hsp70 gene sequencing and MALDI-TOF MS [21].