Based on the Results of PEDV Phylogenetic Analysis of the Most Recent Isolates in China, the Occurrence of Further Mutations in the Antigenic Site S1° and COE of the S Protein Which Is the Target Protein of the Vaccine

Figure 1

Isolation and identification of the PEDV CH/HLJBQL/2022 strain. (a) Assays for PCV2, PDCoV, TGEV, PRRSV, PBoV, PRV, PKV, BVDV, and PEDV were performed sequentially, with the complex number being the singular negative control, using 2000 marker as the standard. (b) Small intestines and their contents, which tested positive for PEDV, were made up as an adsorbent solution, and Vero E6 cells were infected, and it was observed that after shedding of the cells freeze-thawed three times and the mixture was tested. Domesticated CV777 served as a positive control. (c) A. Transmission electron microscopy of Vero E6 cell supernatant infected with CV777. B Transmission electron microscopy of Vero E6 cell supernatant infected with CH/HLJBQL/2022. (d) Immunofluorescence results of mock infected group, isolate infected group, and CV777 infected group. Infected cells were detected using a monoclonal antibody against PEDV N protein and Alexa Fluor 488 conjugated goat anti-mouse IgG and quantified using ImageJ (n = 3, ).

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