Development and Application of an iELISA for the Detection of Antibody against Salmonella Abortusequi

Figure 2

Purification and identification of recombinant FljB protein. (a) SDS–PAGE analysis of purified FljB protein. M, protein marker; lane 1–4, purified protein. (b) Western blotting detection of FljB using an anti-his tag antibody. (c) western blotting detection of FljB using S. Abortusequi positive serum. (d) Western blotting detection of FljB using S. Abortusequi negative serum.