Development and Application of an iELISA for the Detection of Antibody against Salmonella Abortusequi

Table 1

Serum samples employed in this study.

Purpose	Amounts	Name/description	Sample source
Development of iELISA	2	Standard positive and negative serum	Stored in our lab
Cut-off value determination	1030	Clinical healthy equine sera samples verified as negative by TAT and western blotting	Farms in China
Analytical specificity	6	Reference sera against equine herpesvirus 1 (EHV-1), 2 (EHV-2), 3 (EHV-3), equine influenza virus (EIV-prague and EIV-kentucky), and equine arteritis virus (EAV)	NVSL, USA
	8	Sera against equine infectious anemia virus (EIAV), equine herpesvirus (EHV-4 and EHV-7), Streptococcus equi (S. equi), S. Dublin, S. Typhimurium, S. Enteritidis, and Escherichia coli	Stored in our lab
Analytical sensitivity	3	Serums samples verified by TAT as having high (8×), medium (4×) and low (1×) antibody concentrations	Stored in our lab
Diagnostic sensitivity and specificity analysis	176	88 collected from the horses infected with S. Abortusequi in farms 1–3	Farms in China
		88 collected from the healthy horses in farm 4
Comparison of positive detection rates between TAT and iELISA	508	335 from infected horses in farms 5–8	Farms in China
		173 samples from healthy horses in farms 9–13
Serological surveillance	1472	1472 from 51 farms in 18 provinces	Farms in China

NVSL is an abbreviation for “National Veterinary Services Laboratories, Inc.” in USA.