Research Article
Development and Application of an iELISA for the Detection of Antibody against Salmonella Abortusequi
Table 2
Comparison of the performance of FljB iELISA and TAT assays.
| | Location | Source of serum | Clinical symptoms | Vaginal swab | Pathogen identification | Serum samples | TAT/iELISA | Positive detection rate (%) (95% CI) | | (+/+) | (+/−) | (−/+) | (−/−) | TAT | FljB iELISA |
| | Farm 1 | Mares | Abortion | 43 | S. Abortusequi | 43 | 14 | 0 | 28 | 1 | 29.3 (27.56–31.04) | 97.6 (97.49–97.41) |
| | Farm 2 | Mares | Abortion | 20 | S. Abortusequi | 20 | 5 | 0 | 15 | 0 | 25.0 (21.33–28.68) | 100.0 (100) |
| | Farm 3 | Mares | Abortion | 25 | S. Abortusequi | 25 | 15 | 0 | 10 | 0 | 60.0 (57.57–62.43) | 100.0 (100) |
| | Farm 4 | Mares | Normal | 88 | — | 88 | 0 | 0 | 0 | 88 | 0 | 0 |
| | Farm 5 | Mares | Abortion | 5 | S. Abortusequi | 146 | 10 | 0 | 102 | 34 | 6.8 (6.47–7.13) | 76.7 (76.43–76.97) |
| | Farm 6 | Mares | Abortion | 12 | S. Abortusequi | 65 | 20 | 0 | 26 | 19 | 30.8 (29.64–31.96) | 70.8 (70.06–71.54) |
| | Farm 7 | Mares | Abortion | 6 | S. Abortusequi | 57 | 5 | 0 | 46 | 6 | 8.8 (7.87–9.13) | 89.5 (89.16–89.84) | | Foals | Arthroncus | 7 | S. Abortusequi | 43 | 3 | 0 | 21 | 19 | 7.0 (5.88–8.12) | 55.8 (54.30–57.30) |
| | Farm 8 | Mares | Abortion | 4 | S. Abortusequi | 24 | 3 | 0 | 15 | 6 | 12.5 (9.97–15.03) | 75.0 (73.23–76.77) |
| | Farm 9 | Mares | Normal | — | — | 50 | 0 | 1 | 0 | 49 | 2 (1.46–2.54) | 0 |
| | Farm 10 | Stallion | Normal | — | — | 16 | 0 | 0 | 0 | 16 | 0 | 0 |
| | Farm 11 | Mares | Normal | — | — | 26 | 0 | 0 | 1 | 25 | 0 | 3.8 (2.39–5.21) |
| | Farm 12 | Mares | Normal | — | — | 57 | 2 | 0 | 5 | 50 | 3.5 (2.88–4.12) | 12.3 (11.24–13.36) |
| | Farm 13 | Stallion | Normal | — | — | 24 | 0 | 0 | 2 | 22 | 0 | 8.3 (6.14–10.46) |
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