Characterization of the First Case of Classical Scrapie in a Sheep in Tunisia

Figure 1

Hematoxylin and eosin staining (a–c) and PrP^Sc immunohistochemistry (d–h) of brain or retropharyngeal lymph node (i) of the sheep 19/185. Spongiform change of the neuropil (arrows) in the mediodorsal thalamic nucleus (a); spongiosis (arrows) of the neuropil in the deep layers of occipital cortex (b) and intraneuronal vacuoles (arrows) in the dorsal nucleus of the vagus nerve (c). Main PrP^Sc deposition patterns observed in the brain (d–h). Hypoglossal nucleus: perineuronal and intraneuronal (arrows) and synaptic/punctate (arrowheads) in the neuropil (d); intraneuronal accumulation in the reticular formation of medulla oblongata (arrows) (e); stellate deposition in the white matter of the occipital cortex (f); stellate (arrows) and small PrP^Sc deposits (arrowheads) in the molecular and granular layer of the cerebellum, respectively (g); perivascular in the white matter of the occipital cortex (h). Retropharyngeal lymph node (i): granular and punctuate PrP^Sc depositions in the germinal centre of a secondary lymphoid nodule (i) (scale bar for all figures of the panel, 50 µm).

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