Characterization and Evaluation of the Pathogenicity of a Natural Gene-Deleted Transmissible Gastroenteritis Virus in China

Figure 1

Isolation and identification of TGEV SC2021. (a) The microscopic image at 36 hours post-infection. Uninfected cells were the PK-15 cells which treated with DMEM; TGEV SC-Y was the PK-15 cells infected with TGEV SC-Y strain; TGEV SC2021 was the PK-15 cells infected with TGEV SC2021 strain. The black arrow points the CPE. (b) PCR identification results of the isolation supernatant. Lane (M) DNA Marker; lane 1: TGEV positive control; lane 2–4: 5th, 10th, 15th generations of the isolation supernatant; lane 5: TGEV negative control; lane 6: PEDV positive control; lane 7–9: 5th, 10th, 15th generations of the isolation supernatant; lane 10: PEDV negative control; lane 11: RV positive control; lane 12–14: 5th, 10th, 15th generations of the isolation supernatant; lane 15: RV negative control; lane 16: PDCoV positive control; lane 17–19: 5th, 10th, 15th generations of the isolation supernatant; lane 20: PDCoV negative control. (c) Growth kinetics of TGEV SC2021 and SC-Y with different time in PK-15 cells.

(a)

(b)

(c)