Attenuation of a Highly Pathogenic Porcine Deltacoronavirus Strain CZ2020 by a Serial Passage In Vitro

Figure 8

Determination of cytokines in the ileum tissues. The cytokines, including (a) TNF-α, (b) IL-1β, (c) IL-6, (d) IFN-α, (e) IFN-λ1, and (f) IFN-λ3, in the ileum samples were determined by using the SYBR green-based RT-qPCR. The experiments were performed in triplicate and data are expressed as the mean ± standard deviations for triplicate samples. An unpaired two-tailed t-test was used to determine the statistical significance. ; ; ; ns, no significance.

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