Attenuation of a Highly Pathogenic Porcine Deltacoronavirus Strain CZ2020 by a Serial Passage In Vitro

Table 1

Primer sequences used in this study.

Primer ID	Sequence (5′–3′)
PDCoV-NF	TGG​CAA​TGG​AGT​TCC​GCT​TA
PDCoV-NR	GGG​TAT​CAT​TAG​GAG​GGA​GTT
PDCoV-ks-N	FAM-TGGCACAGGTCCCAGAGGAAATCT-BHQ1
IFN-α-F	TTC​TGC​ACT​GGA​CTG​GAT​C
IFN-α-R	TCT​GTG​GAA​GTA​TTT​CCT​CAC​AG
IFN-λ1-F	GGT​GTT​GGC​GAC​TGT​GAT​G
IFN-λ1-R	GAT​TGG​AAC​TGG​CCC​ATG​TG
IFN-λ3-F	TTG​GCC​CAG​TTC​AAG​TCT​CT
IFN-λ3-R	GAG​CTG​CAG​TTC​CAG​TCC​TC
IL-6-F	CTG​GCA​GAA​AAC​AAC​CTG​AAC​C
IL-6-R	TGA​TTC​TCA​TCA​AGC​AGG​TCT​CC
IL-1β-F	AAC​GTG​CAG​TCT​ATG​GAG​T
IL-1β-R	GAA​CAC​CAC​TTC​TCT​CTT​CA
TNF-α-F	AAC​CTC​AGA​TAA​GCC​CGT​CG
TNF-α-R	ACC​ACC​AGC​TGG​TTG​TCT​TT