Re-Emergence of BTV-4 in Sheep Farms in Kosovo, 2020: A Retrospective Study

Table 1

The percent nucleotide sequence (nt%) identity for individual segments of BTV-KOS2020/01 and BTV-KOS2020/02 against closely related sequences from GenBank and a newly sequenced isolate from the 2014 BTV-4 outbreak in Kosovo (KOS2014/01).

Segment	Length (bp)	GenBank accession	Strain ID	Country, isolation date	KOS2020/01 nt% identity	KOS2020/02 nt% identity
1	3944	MT879201	MKD2020/06	North Macedonia, 2020	99.95	99.95
		MG944817	BTV-4/17-15	France, 2017	99.77	99.77
		MT879211	GRE2014/08	Greece, 2014	99.72	99.72
		KP268814	HUN2014	Hungary, 2014	99.67	99.67
		OP186416	KOS2014/01	Kosovo, 2014	99.62	99.67
2	2926	MT879202	MKD2020/06	North Macedonia, 2020	99.93	99.97
		MG944818	BTV-4/17-15	France, 2017	99.76	99.79
		MT879212	GRE2014/08	Greece, 2014	99.73	99.76
		KP268815	HUN2014	Hungary, 2014	99.66	99.69
		OP186417	KOS2014/01	Kosovo, 2014	99.72	99.76
3	2772	MT879203	MKD2020/06	North Macedonia, 2020	99.96	100
		MG944819	BTV-4/17-15	France, 2017	99.86	99.89
		MT879213	GRE2014/08	Greece, 2014	99.82	99.86
		KP268816	HUN2014	Hungary, 2014	99.78	99.82
		OP186418	KOS2014/01	Kosovo, 2014	99.82	99.86
4	1981	MT879204	MKD2020/06	North Macedonia, 2020	99.95	100
		MG944820	BTV-4/17-15	France, 2017	99.80	99.85
		MT879214	GRE2014/08	Greece, 2014	99.85	99.90
		KP268817	HUN2014	Hungary, 2014	99.85	99.90
		OP186419	KOS2014/01	Kosovo, 2014	99.85	99.90
5	1774	MT879205	MKD2020/06	North Macedonia, 2020	100	100
		MG944821	BTV-4/17-15	France, 2017	99.83	99.83
		MT879215	GRE2014/08	Greece, 2014	99.94	99.94
		KP268818	HUN2014	Hungary, 2014	99.89	99.89
		OP186420	KOS2014/01	Kosovo, 2014	99.89	99.77
6	1637	MT879206	MKD2020/06	North Macedonia, 2020	99.94	100
		MG944822	BTV-4/17-15	France, 2017	99.50	99.57
		MT879216	GRE2014/08	Greece, 2014	99.33	99.39
		KP268819	HUN2014	Hungary, 2014	99.33	99.39
		OP186421	KOS2014/01	Kosovo, 2014	99.45	99.51
7	1156	MT879207	MKD2020/06	North Macedonia, 2020	99.91	100
		MG944823	BTV-4/17-15	France, 2017	99.74	99.83
		MT879217	GRE2014/08	Greece, 2014	99.83	99.91
		KP268820	HUN2014	Hungary, 2014	99.91	100
		OP186422	KOS2014/01	Kosovo, 2014	99.91	100
8	1125	MT879208	MKD2020/06	North Macedonia, 2020	99.91	100
		MG944824	BTV-4/17-15	France, 2017	99.63	99.73
		MT879218	GRE2014/08	Greece, 2014	99.64	99.73
		KP268821	HUN2014	Hungary, 2014	99.64	99.73
		OP186423	KOS2014/01	Kosovo, 2014	99.64	99.73
9	1049	MT879209	MKD2020/06	North Macedonia, 2020	99.90	100
		MG944825	BTV-4/17-15	France, 2017	99.60	99.70
		MT879219	GRE2014/08	Greece, 2014	99.52	99.62
		KP268822	HUN2014	Hungary, 2014	99.52	99.62
		OP186424	KOS2014/01	Kosovo, 2014	99.62	99.71
10	822	MT879210	MKD2020/06	North Macedonia, 2020	100	100
		MG944826	BTV-4/17-15	France, 2017	99.88	99.88
		MT879220	GRE2014/08	Greece, 2014	99.51	99.51
		KP268823	HUN2014	Hungary, 2014	99.64	99.64
		OP186425	KOS2014/01	Kosovo, 2014	99.51	99.51

indicates <100% query coverage.