Characterization of a Highly Virulent Noncytopathic Bovine Viral Diarrhea Virus 2b Isolate Detected in Cattle in Inner Mongolia, China

Figure 1

Cytopathic effects and phylogenetic analysis of the BVDVNM21 strain. (a) MDBK cells were infected with BVDVNM21 strain. (b) MDBK cells were infected with BVDVSD1301 strain (positive control). (c) Mock MDBK cells. MDBK cells were inoculated with (d) the BVDVNM21 and (e) the BVDVSD1301, and specific fluorescence was detected, but (f) uninfected cells did not show fluorescence. Phylogenetic tree analysis of (g) the complete sequence, (h) 5′UTR, (i) Npro, and E2 (j) was prepared using the neighbor-joining method by the software MEGA 7.0.

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